Best Lab Cell Culture Flasks of 2025

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Lab cell culture flasks are essential tools for cell culture research in laboratories. These flasks are designed to provide a controlled environment for the growth and proliferation of cells. Made from high-quality materials such as borosilicate glass or polystyrene, these flasks are sterile and provide an optimal surface for cell attachment and growth. They come in various sizes, including small, medium, and large, to accommodate different cell culture volumes. With their leak-proof caps and ergonomic design, lab cell culture flasks ensure a safe and convenient environment for cell culture experiments.
At a Glance: Our Top Picks
Top 10 Lab Cell Culture Flasks
Nest 708003 Cell Culture Flask
The Nest Cell Culture Flask is designed for a variety of cell growth needs, with sizes ranging from 25cm² to 225cm². This versatility allows you to choose the perfect flask for your specific experiments. You can select between tissue culture treated or non-treated options, giving you the flexibility to meet different cell culture requirements. The flasks come with either a vent cap or a plug seal cap, ensuring that you have the right sealing option to prevent contamination while allowing for gas exchange. This thoughtful design enhances convenience and efficiency in your lab work.
Nest Scientific Polystyrene Cell Culture Flask
The Nest Cell Culture Flask is designed for optimal lab use. It comes packaged in sterile, zip-sealable bags, ensuring that you maintain a contamination-free environment. Made from high-quality, clear polystyrene, you'll appreciate the frosted writing and clear graduations, which make it easy to measure and label your samples. The notched bottom allows for secure stacking without slipping, enhancing your workspace efficiency. Additionally, each flask is sterilized using E Beam technology, providing you with peace of mind regarding cleanliness and safety in your experiments.
Celltreat 229510 Suspension Culture Flask
Celltreat Suspension Flask is a sterile lab cell culture flask designed for suspension cultures. It is not tissue culture treated and has a hydrophobic surface, which prevents cell attachment. The flask comes with a 0.22μm hydrophobic membrane vent cap, allowing for gas exchange. It is packaged in zip-closure bags, ensuring sterility. With its non-tissue culture treated surface, this flask is ideal for suspension cultures where cell attachment is not desired. The hydrophobic membrane vent cap facilitates gas exchange, ensuring optimal conditions for cell growth. The sterile packaging provides convenience and peace of mind, saving time on sterilization procedures. The Celltreat Suspension Flask is a reliable and convenient option for lab cell culture applications.
SPL T25 Cell Culture Flask
The SPL T25 Flask is designed with an ergonomic shape that makes it easy for you to handle during your lab work. Its short and wide neck, combined with an angled design, allows for effortless access to the contents, ensuring you can easily perform your tasks. The flask also boasts excellent stackability, which helps you save valuable storage space in your lab. Additionally, it is non-pyrogenic, non-cytotoxic, and free from DNase, RNase, and human DNA, providing you with peace of mind when conducting sensitive experiments.
NEST Scientific 731002 5-Layer Cell Culture Flask
Nest Scientific 731002 5-Layer Cell Culture Flask is a high-quality lab flask designed for cell culture. It features vacuum plasma TC treatment, which ensures the best cell attachment for successful experiments. The flask is sterilized by E-beam, making it safe for use in sensitive laboratory environments. It is also non-pyrogenic and dnasernase free, providing a clean and reliable platform for cell culture. The flask comes with both a plug seal cap and vent cap options, allowing users to choose the appropriate sealing method based on their specific needs. With its exceptional features, NEST 5-Layer Cell Culture Flask offers convenience and reliability for all your cell culture experiments.
Celltreat 229520 250mL Suspension Flask
The Celltreat Suspension Flask is designed for specific laboratory applications where cell attachment is not required. Its hydrophobic surface prevents cells from adhering, making it ideal for suspension cultures. The flask features a 0.22μm hydrophobic membrane vent cap, which facilitates essential gas exchange for your cell cultures. Additionally, each flask is gamma irradiated and packaged in sterile zip-closure bags, ensuring that you receive a clean and safe product ready for use. This combination of features provides convenience and reliability for researchers working in cell culture environments.
Advangene Polystyrene Culture Flask
Introducing the Advangene Polystyrene Culture Flask, a high-quality lab cell culture flask designed to meet your scientific needs. Manufactured under ISO9001 by ADVANGENE, a reputable US brand, this non-treated polystyrene flask offers a working volume of 175 ml for maximum growth of your tissue cultures. The flask features a graduation range of 20 ml to 175 ml, with 25 ml subdivisions, allowing for precise measurements. With its uniform thickness and optical clarity, the flask walls provide excellent visibility. The canted-neck design ensures easy access, while the flask ridges enable stable stacking. Trust the Advangene Polystyrene Culture Flask to provide convenience and reliability for your cell culture experiments.
CryoKing Cell Culture Flasks 175cm²
The CryoKing Culture Flasks are designed to enhance your cell culture experience. The filter cap allows for optimal gas exchange, which is essential for healthy cell growth and metabolism. You'll appreciate the short, wide neck design that makes it easy to access your cultures, whether you’re using a cell scraper or inoculating loops. The advanced TC treatment improves cell adhesion, boosting your cultivation efficiency. Plus, the high-quality polystyrene construction offers excellent transparency, making it simple to monitor cell growth under a microscope.
Corning U-Shaped Cell Culture Flask
The Corning U-Shaped Flask offers a generous 75 cm² area for cell growth, making it ideal for your laboratory needs. Its design minimizes corners, allowing for easy scraping of cells, which can enhance your efficiency during experiments. You will also appreciate the improved grip, giving you better access to the cap when handling the flask. Additionally, the flask features a pipet divot, enabling you to remove liquids conveniently with a pipet, saving you time and effort in your workflow.
CryoKing 75cm² Cell Culture Flask
The CryoKing Cell Culture Flask offers several key features to enhance your cell culture experience. Its plug cap design effectively blocks outside air and impurities, helping you maintain a stable and pure culture environment. The flask's short and wide angled neck allows easy access, making it simple to use with tools like cell scrapers or inoculating loops. Additionally, the advanced TC treatment improves cell attachment and growth, increasing your cultivation efficiency. With its high transparency, you can easily observe cell growth through a microscope, ensuring you stay informed about your cultures.
Frequently Asked Questions (FAQs)
1. Why T25 flask is used in cell culture?
T25 flasks are used in cell culture because they are composed of cell culture-treated polystyrene and have a surface treated with proprietary Nunclon Delta surface. This surface supports optimal growth and attachment of cells to the growth surface. The filtered caps of T25 flasks allow for constant airflow, while the vent/close caps allow for sufficient gas exchange.
2. What is the difference between T25 and T75 flasks?
The main difference between T25 and T75 flasks is the surface area. T25 flasks have a surface area of 25 cm2, while T75 flasks have a surface area of 75 cm2. Both types of flasks can be provided with plug caps or filter caps and are surface treated for cell culture. They are sterilized by gamma irradiation.
3. What are T75 flasks for adherent cells?
T75 flasks with poly-d-lysine coating or collagen I coatings are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface. These coatings facilitate better attachment and growth of the cells on the flask surface.
4. What is a T225 flask?
A T225 flask is a type of cell culture flask that is composed of cell culture-treated polystyrene and has a surface treated with proprietary Nunclon Delta surface. Similar to other flasks, T225 flasks support optimal growth and attachment of cells to the growth surface.
5. How many hek293 cells are in a T75 flask?
In a T75 flask, the approximate cell number for 100% confluence for the HEK293 cell line is 1 × 107 cells. The specific media formulations for these cells can be found in Section 1.
6. How many cells can I seed in a T25 flask?
For a T25 flask, it is recommended to seed approximately 1.7 x 105 cells per cm2 of flask surface area. This translates to around 6.6 x 103 cells per cm2 or 4.95 x 105 cells for a T75 cm2 flask. If there is any remaining cell suspension, it should be divided evenly among all the flasks.
7. How many cells can a T75 flask hold?
A T75 flask can hold approximately 1.2E+6 cells. This capacity is based on the optimal cell growth and confluence achieved in the flask.
8. What is a T175 flask?
A T175 flask is a type of cell culture flask composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surfaces. These flasks support optimal growth and attachment of cells to the growth surface. They are designed to provide enhanced cell performance with the Nunclon Delta surfaces.
During our lab cell culture flask research, we found 24 lab cell culture flask products and shortlisted 10 quality products. We collected and analyzed 2,254 customer reviews through our big data system to write the lab cell culture flasks list. We found that most customers choose lab cell culture flasks with an average price of $120.31.
The lab cell culture flasks are available for purchase. We have researched hundreds of brands and picked the top brands of lab cell culture flasks, including Nest, Celltreat, SPL Life Sciences, Advangene, CryoKing. The seller of top 1 product has received honest feedback from 131 consumers with an average rating of 4.5.

Brian Howie is a content writer and senior industrial designer. After graduation from Westminster College, he has been engaged in product design and manufacturing for 18 years. Brian Howie has always found great pleasure in designing industrial products and sharing his ideas and experience online.